Lolu cwaningo lubonisa ukuthi isikhunta esihlobene nezimpande i-Kosakonia oryziphila NP19, esihlukaniswe nezimpande zelayisi, siyisidakamizwa esikhuthaza ukukhula kwezitshalo esikhuthaza ukukhula kwezitshalo kanye ne-biochemical ukulawula ukuqhuma kwelayisi. Ukuhlolwa kwe-in vitro kwenziwe emaqabunga amasha ezithombo zelayisi ezinephunga elimnandi i-Khao Dawk Mali 105 (KDML105). Imiphumela ibonise ukuthi i-NP19 ivimbele ngempumelelo ukuhluma kwe-rice blast fungal conidia. Ukutheleleka kwe-fungal kwavinjelwa ngaphansi kwezimo ezintathu ezahlukene zokwelapha: ukugonywa kwelayisi nge-NP19 kanye ne-fungal conidia; ukugonywa kweqabunga ngasikhathi sinye nge-NP19 kanye ne-fungal conidia; kanye nokugonywa kweqabunga nge-fungal conidia kulandelwe ukwelashwa kwe-NP19 ngemva kwamahora angu-30. Ngaphezu kwalokho, i-NP19 yehlise ukukhula kwe-fungal hyphal ngo-9.9–53.4%. Ekuhlolweni kwembiza, i-NP19 yandise imisebenzi ye-peroxidase (POD) kanye ne-superoxide dismutase (SOD) ngo-6.1% kuya ku-63.0% kanye no-3.0% kuya ku-67.7%, ngokulandelana, okubonisa izindlela zokuzivikela zezitshalo ezithuthukisiwe. Uma kuqhathaniswa nokulawulwa kwe-NP19 okungathelelekile, izitshalo zerayisi ezinegciwane le-NP19 zibonise ukwanda kokuqukethwe kombala ngo-0.3%–24.7%, inani lokusanhlamvu okugcwele nge-panicle ngo-4.1%, isivuno sokusanhlamvu okugcwele ngo-26.3%, inkomba yobuningi besivuno ngo-34.4%, kanye nokuqukethwe kwe-aromatic compound 2-acetyl-1-pyrroline (2AP) ngo-10.1%. Ezitshalweni zerayisi ezinegciwane le-NP19 kanye ne-blast, ukwanda kwaba ngu-0.2%–49.2%, 4.6%, 9.1%, 54.4%, kanye no-7.5%, ngokulandelana. Ukuhlolwa kwasensimini kubonise ukuthi izitshalo zerayisi eziqoqwe kanye/noma ezifakwe i-NP19 zibonise ukwanda kwenani lokusanhlamvu okugcwele nge-panicle ngayinye ngo-15.1–27.2%, isivuno sokusanhlamvu okugcwele ngo-103.6–119.8%, kanye nokuqukethwe kwe-2AP ngo-18.0–35.8%. Lezi zitshalo zerayisi zibonise nomsebenzi ophezulu we-SOD (6.9–29.5%) uma kuqhathaniswa nezitshalo zerayisi ezinegciwane lokuqhuma ezingafakwanga i-NP19. Ukusetshenziswa kwamaqabunga e-NP19 ngemuva kokutheleleka kunciphisa ukuqhubekela phambili kwesilonda. Ngakho-ke, i-K. oryphila NP19 iboniswe ukuthi iyindlela yokukhula kwesitshalo ekhuthaza i-bioagent kanye ne-biopesticide yokulawula ukuqhuma kwerayisi.
Kodwa-ke, ukusebenza kahle kwemithi yokubulala isikhunta kuthonywa yizici eziningi, okuhlanganisa ukwakheka, isikhathi nendlela yokusebenzisa, ubukhali besifo, ukusebenza kahle kwezinhlelo zokubikezela izifo, kanye nokuvela kwezinhlobo ezimelana nemithi yokubulala isikhunta. Ngaphezu kwalokho, ukusetshenziswa kwemithi yokubulala isikhunta yamakhemikhali kungabangela ubuthi obusele emvelweni futhi kube yingozi empilweni yabasebenzisi.
Ekuhlolweni kwembiza, imbewu yelayisi yahlanzwa phezulu futhi yahluma njengoba kuchaziwe ngenhla. Yabe isihlwanyelwa nge-K. oryphila NP19 yabe isitshalwa emathileyi ezithombo. Izithombo zafakwa esikhumbeni izinsuku ezingama-30 ukuze kuvele izithombo zelayisi. Izithombo zabe sezitshalwa emabhodweni. Ngesikhathi senqubo yokufakelwa, izitshalo zelayisi zavundiswa ukuze zilungiselele ukutheleleka ngesikhunta esibangela ukuqhuma kwelayisi nokuhlola ukumelana kwazo.
Ekuhlolweni kwensimu, imbewu ehlumekile etheleleke nge-Aspergillus oryzae NP19 yelashwa kusetshenziswa indlela echazwe ngenhla futhi yahlukaniswa ngamaqembu amabili: imbewu etheleleke nge-Aspergillus oryzae NP19 (RS) kanye nembewu engathelelekile (US). Imbewu ehlumekile yatshalwa emathileyi anenhlabathi ehlanziwe (ingxube yenhlabathi, igobolondo lelayisi elishisiwe, kanye nomquba ngesilinganiso esingu-7:2:1 ngesisindo) futhi yagcinwa izinsuku ezingu-30.
I-oryziphila conidial suspension yengezwe elayisini le-R futhi ngemva kwamahora angu-30 okufukamela, kwanezelwa ama-2 μl e-K. oryziphila NP19 endaweni efanayo. Zonke izitsha ze-Petri zafakwa e-incubator kuma-25°C ebumnyameni amahora angu-30 zase zifakwa e-incubator ngaphansi kokukhanya okuqhubekayo. Iqembu ngalinye laphindwa kathathu. Ngemva kwamahora angu-72 okufukamela, izingxenye zezitshalo zahlolwa futhi zenziwa i-scanning electron microscopy. Kafushane, izingxenye zezitshalo zaqiniswa ku-phosphate-buffered saline equkethe i-2.5% (v/v) glutaraldehyde futhi zacwiliswa ochungechungeni lwezixazululo ze-ethanol. Amasampula omiswe nge-critical-point nge-carbon dioxide, abese egcotshwa ngegolide futhi abonwa ngaphansi kwe-scanning electron microscope imizuzu engu-15.
Isikhathi sokuthunyelwe: Okthoba-13-2025