Amakhowe amakhulu anesethi ecebile nehlukahlukene yama-metabolites asebenzayo futhi abhekwa njengemithombo ebalulekile yezinto eziphilayo. I-Phellinus igniarius iyikhowe elikhulu elivame ukusetshenziswa kokubili ngezinhloso zokwelapha nezokudla, kodwa ukuhlukaniswa kwalo kanye negama lesiLatini kusalokhu kuphikisana. Besebenzisa ukuhlaziywa kokuhlelwa kwezigaba zezakhi zofuzo eziningi, abacwaningi baqinisekisile ukuthi i-Phellinus igniarius kanye nezinhlobo ezifanayo ziyingxenye yohlobo olusha futhi basungula uhlobo lwe-Sanghuangporus. Ikhowe le-honeysuckle i-Sanghuangporus lonicericola lingenye yezinhlobo ze-Sanghuangporus ezikhonjwe emhlabeni jikelele. I-Phellinus igniarius idonsele ukunaka okukhulu ngenxa yezakhiwo zayo zokwelapha ezahlukahlukene, kufaka phakathi ama-polysaccharides, ama-polyphenols, ama-terpenes, nama-flavonoid. Ama-Triterpenes ayizinhlanganisela ezibalulekile ezisebenzayo kwezokwelapha zalolu hlobo, ezibonisa imisebenzi ye-antioxidant, antibacterial, kanye ne-antitumor.
Ama-Triterpenoid anamandla amakhulu okusetshenziswa kwezentengiselwano. Ngenxa yokungatholakali kwemithombo yasendle ye-Sanghuangporus emvelweni, ukuthuthukisa ngempumelelo ukusebenza kwayo kwe-biosynthetic kanye nokukhiqiza kubaluleke kakhulu. Njengamanje, kuye kwenziwa intuthuko ekukhuliseni ukukhiqizwa kwama-metabolites ahlukahlukene esibili e-Sanghuangporus ngokusebenzisa ama-inducers amakhemikhali ukulawula amasu okubilisa angaphansi komhlaba. Isibonelo, ama-polyunsaturated fatty acid, ama-fungal elicitors11 kanye nama-phytohormones (kufaka phakathi i-methyl jasmonate kanye ne-salicylic acid14) kuye kwabonakala kukhulisa ukukhiqizwa kwe-triterpenoid e-Sanghuangporus. Abalawuli bokukhula kwezitshalo(Ama-PGR)ingalawula ukwenziwa kwe-biosynthesis kwama-metabolite esibili ezitshalweni. Kulolu cwaningo, kuhlolwe i-PBZ, umlawuli wokukhula kwezitshalo osetshenziswa kabanzi ukulawula ukukhula kwezitshalo, isivuno, ikhwalithi kanye nezici zomzimba. Ikakhulukazi, ukusetshenziswa kwe-PBZ kungathonya indlela yokwenza i-terpenoid ezitshalweni. Ukuhlanganiswa kwama-gibberellins ne-PBZ kwandise okuqukethwe kwe-quinone methide triterpene (QT) ku-Montevidia floribunda. Ukwakheka kwendlela ye-terpenoid yamafutha e-lavender kwashintshwa ngemva kokwelashwa nge-400 ppm PBZ. Kodwa-ke, akukho mibiko ngokusetshenziswa kwe-PBZ kumakhowe.
Ngaphezu kwezifundo ezigxile ekwandeni kokukhiqizwa kwe-triterpene, ezinye izifundo ziphinde zacacisa izindlela zokulawula ze-triterpene biosynthesis ku-Moriformis ngaphansi kwethonya lama-chemical inducers. Njengamanje, izifundo zigxile ekuguqulweni kwamazinga okubonakaliswa kwezakhi zofuzo zesakhiwo ezihlobene ne-triterpene biosynthesis endleleni ye-MVA, okuholela ekwandeni kokukhiqizwa kwe-terpenoid.12,14 Kodwa-ke, izindlela ezisekela la ma-gene ezaziwayo zesakhiwo, ikakhulukazi izici zokubhala ezilawula ukuvezwa kwawo, azikacaci ezindleleni zokulawula ze-triterpene biosynthesis ku-Moriformis.
Kulolu cwaningo, kuhlolwe imiphumela yokugxila okuhlukene kwabalawuli bokukhula kwezitshalo (ama-PGR) ekukhiqizweni kwe-triterpene kanye nokukhula kwe-mycelial ngesikhathi sokubiliswa kwe-honeysuckle (S. lonicericola) ngaphansi kwamanzi. Ngemva kwalokho, kwasetshenziswa i-metabolomics kanye ne-transcriptomics ukuhlaziya ukwakheka kwe-triterpene kanye namaphethini okubonakaliswa kwezakhi zofuzo ahilelekile ku-biosynthesis ye-triterpene ngesikhathi sokwelashwa kwe-PBZ. Idatha yokulandelana kwe-RNA kanye ne-bioinformatics yathola ngokwengeziwe i-target transcription factor ye-MYB (SlMYB). Ngaphezu kwalokho, kwakhiqizwa ama-mutant ukuqinisekisa umphumela wokulawula we-SlMYB gene ku-biosynthesis ye-triterpene kanye nokuhlonza izakhi zofuzo ezingaba khona. Ukuhlolwa kwe-electrophoretic mobility shift assays (EMSA) kwasetshenziswa ukuqinisekisa ukusebenzisana kwe-SlMYB protein nabagqugquzeli bezakhi zofuzo ze-SlMYB. Ngamafuphi, inhloso yalolu cwaningo kwakuwukukhuthaza i-triterpene biosynthesis kusetshenziswa i-PBZ nokuhlonza i-MYB transcription factor (SlMYB) elawula ngqo izakhi zofuzo ze-triterpene biosynthetic okuhlanganisa i-MVD, i-IDI, kanye ne-FDPS ku-S. lonicericola ekuphenduleni ukuqaliswa kwe-PBZ.
Ukungeniswa kwe-IAA kanye ne-PBZ kwandise kakhulu ukukhiqizwa kwe-triterpenoid ku-honeysuckle, kodwa umphumela wokungeniswa kwe-PBZ wawubonakala kakhulu. Ngakho-ke, i-PBZ yatholakala iyisikhuthazi esihle kakhulu ekugxilweni okwengeziwe okungu-100 mg/L, okufanele kufundwe kabanzi.
Isikhathi sokuthunyelwe: Agasti-19-2025