Amakhowe amakhulu anesethi ecebile nehlukahlukene yama-metabolites e-bioactive futhi athathwa njengemithombo ebalulekile yezinto eziphilayo. I-Phellinus igniarius iyikhowe elikhulu ngokwesiko elisetshenziselwa kokubili izinjongo zokwelapha nokudla, kodwa ukuhlukaniswa kwalo negama lesiLatini kusalokhu kuyimpikiswano. Besebenzisa ukuhlaziywa kokuqondanisa kwengxenye ye-multigene, abacwaningi baqinisekisile ukuthi i-Phellinus igniarius nezinhlobo ezifanayo ziyingxenye yohlobo olusha futhi basungula uhlobo lwe-Sanghuangporus. Ikhowe le-honeysuckle i-Sanghuangporus lonicericola ingenye yezinhlobo ze-Sanghuangporus ezihlonziwe emhlabeni wonke. I-Phellinus igniarius idonse ukunakwa okukhulu ngenxa yezakhiwo zayo zokwelapha ezahlukahlukene, kufaka phakathi ama-polysaccharides, ama-polyphenols, ama-terpenes, nama-flavonoids. I-Triterpenes iyinhlanganisela eyinhloko yemithi yalolu hlobo, ebonisa imisebenzi ye-antioxidant, antibacterial, kanye ne-antitumor.
I-Triterpenoids inamandla amakhulu okusetshenziswa kwezentengiso. Ngenxa yokungatholakali kwemithombo yasendle ye-Sanghuangporus ngokwemvelo, ukuthuthukisa ngempumelelo ukusebenza kahle kwayo kwe-biosynthetic kanye nesivuno kubaluleke kakhulu. Njengamanje, inqubekelaphambili yenziwe ekuthuthukiseni ukukhiqizwa kwama-metabolite esibili e-Sanghuangporus ngokusebenzisa ama-inducers amakhemikhali ukuze kulawulwe amasu okuvubela angaphansi kwamanzi. Isibonelo, ama-acids anamafutha e-polyunsaturated, ama-elicitor fungal11 nama-phytohormones (okuhlanganisa i-methyl jasmonate ne-salicylic acid14) aboniswe ukwandisa ukukhiqizwa kwe-triterpenoid e-Sanghuangporus. Izilawuli zokukhula kwezitshalo(PGRs)ingalawula i-biosynthesis yama-metabolites esibili ezitshalweni. Kulolu cwaningo, i-PBZ, isilawuli sokukhula kwezitshalo esisetshenziswa kabanzi ukulawula ukukhula kwezitshalo, isivuno, ikhwalithi kanye nezici zomzimba, yaphenywa. Ikakhulukazi, ukusetshenziswa kwe-PBZ kungathonya indlela ye-terpenoid biosynthetic ezitshalweni. Inhlanganisela yama-gibberellins ne-PBZ inyuse okuqukethwe kwe-quinone methide triterpene (QT) eMontevidia floribunda.Ukwakheka kwendlela ye-terpenoid yamafutha e-lavender kwashintshwa ngemva kokwelashwa nge-400 ppm PBZ. Nokho, ayikho imibiko ngokusetshenziswa kwe-PBZ kumakhowe.
Ngaphezu kwezifundo ezigxile ekwandeni kokukhiqizwa kwe-triterpene, ezinye izifundo ziphinde zacacisa izindlela zokulawula i-triterpene biosynthesis e-Moriformis ngaphansi kwethonya lama-inducers amakhemikhali. Njengamanje, izifundo zigxile ekuguqulweni kwamazinga enkulumo yezakhi zofuzo zesakhiwo ezihlobene ne-triterpene biosynthesis endleleni ye-MVA, okuholela ekwandeni kokukhiqizwa kwe-terpenoid.12,14 Nokho, izindlela ezingaphansi kwalezi zakhi zofuzo ezaziwayo, ikakhulukazi izici zokuloba ezilawula ukukhuluma kwazo, zihlala zingacacile ku-triterpenesis biosynthesis.
Kulolu cwaningo, imiphumela yokugxila okuhlukene kwezilawuli zokukhula kwezitshalo (PGRs) ekukhiqizeni i-triterpene kanye nokukhula kwe-mycelial ngesikhathi sokuvutshelwa okungaphansi kwamanzi kwe-honeysuckle (S. lonicericola) yaphenywa. Kamuva, i-metabolomics kanye ne-transcriptomics yasetshenziselwa ukuhlaziya ukubunjwa kwe-triterpene kanye namaphethini wokuveza izakhi zofuzo ezihilelekile ku-triterpene biosynthesis ngesikhathi sokwelashwa kwe-PBZ. Ukulandelana kwe-RNA kanye nedatha ye-bioinformatics iphinde yahlonza isici esiqondiwe se-MYB (SlMYB). Ngaphezu kwalokho, ama-mutants akhiqizwa ukuze kuqinisekiswe umphumela wokulawula wofuzo lwe-SlMYB ku-triterpene biosynthesis futhi kuhlonzwe izakhi zofuzo ezingase zibe khona. Ama-Electrophoretic mobility shift assays (EMSA) asetshenziselwa ukuqinisekisa ukusebenzisana kweprotheni ye-SlMYB nabathuthukisi bezakhi zofuzo eziqondiwe ze-SlMYB. Kafushane, inhloso yalolu cwaningo bekuwukugqugquzela i-triterpene biosynthesis kusetshenziswa i-PBZ nokuhlonza i-MYB transcription factor (SlMYB) elawula ngokuqondile izakhi zofuzo ze-triterpene biosynthetic ezihlanganisa i-MVD, IDI, ne-FDPS ku-S. lonicericola ekuphenduleni ukungeniswa kwe-PBZ.
Ukufakwa kokubili kwe-IAA kanye ne-PBZ kwandisa kakhulu ukukhiqizwa kwe-triterpenoid ku-honeysuckle, kodwa umphumela wokungeniswa kwe-PBZ wabonakala kakhulu. Ngakho-ke, i-PBZ itholwe iyi-inducer engcono kakhulu ekuhlanganiseni okwengeziwe kwe-100 mg/L, okumele kuqhutshekwe nocwaningo.
Isikhathi sokuthumela: Aug-19-2025