Abacwaningi abavela eMnyangweni we-Biochemistry e-Indian Institute of Sciences (IISc) bathole indlela ekade ifunwayokulawula ukukhula kwezitshalo zasendulo zomhlaba njenge-bryophytes (iqembu elihlanganisa ama-mosses nama-liverwort) ezagcinwa ezitshalweni eziqhakaza kamuva.
Lolu cwaningo, olushicilelwe kumagazini i-Nature Chemical Biology, lugxile ekulawulweni okungekona okwe-canonical kwamaprotheni e-DELLA, umlawuli wokukhula oyinhloko ovimbela ukuhlukaniswa kwamaseli kuma-embryophyte (izitshalo zomhlaba).
Ngokuthakazelisayo, ama-bryophyte, izitshalo zokuqala ukuvela emhlabeni eminyakeni ecishe ibe yizigidi ezingu-500 edlule, awanawo ama-receptor e-GID1, naphezu kokukhiqiza i-phytohormone GA. Lokhu kuphakamisa umbuzo wokuthi ukukhula nokuthuthukiswa kwalezi zitshalo zokuqala zomhlaba kwakulawulwa kanjani.
Besebenzisa i-liverwort Marchantia polymorpha njengohlelo lwemodeli, abacwaningi bathole ukuthi lezi zitshalo zokuqala zisebenzisa i-enzyme ekhethekile, i-MpVIH, ekhiqiza i-cellular messenger inositol pyrophosphate (InsP₈), ukuze iphule i-DELLA ngaphandle kokubandakanyeka kwe-i-gibberellic acid.
Abacwaningi basebenzise uhlelo lwe-CRISPR-Cas9 ukuze basuse i-gene ebhala i-enzyme ye-VIH, okuqinisekisa indima yayo. Izitshalo ezingenayo i-VIH esebenzayo zibonise amaphutha amakhulu okukhula kanye nokukhubazeka kokwakheka, njengamaqabunga aqinile, ukukhula okuphazamisekile kwe-radial, kanye nokuntuleka kwama-calyxes. La maphutha aqedwa ngokushintsha i-genome yesitshalo ukuze kukhiqizwe ukuphela okukodwa kuphela (i-N-terminus) ye-enzyme ye-VIH. Besebenzisa amasu athuthukile e-chromatography, ithimba lithole ukuthi i-N-terminus iqukethe isizinda se-kinase esikhuthaza ukukhiqizwa kwe-InsP₈.
Abacwaningi bathole ukuthi i-DELLA ingenye yezinhloso zamaseli ze-VIH kinase. Ngaphezu kwalokho, baqaphele ukuthi izinhlobo zezitshalo ezingenayo i-MpVIH zifana nezezitshalo ze-M. polymorpha ezinokwanda kokubonakaliswa kwe-DELLA.
"Kuleli qophelo, besijabule kakhulu ukuqonda ukuthi ukuzinza noma umsebenzi we-DELLA uyathuthukiswa yini ezitshalweni ezingenayo i-MpVIH," kusho uPriyanshi Rana, umbhali wokuqala kanye nomfundi ophothule eqenjini locwaningo likaLahey. Ngokuvumelana nombono wabo, abacwaningi bathole ukuthi ukuvinjelwa kwe-DELLA kungabuyisela kakhulu ukukhula okunephutha kanye nentuthuko yezitshalo eziguquliwe ze-MpVIH. Le miphumela iphakamisa ukuthi i-VIH kinase ilawula kabi i-DELLA, ikhuthaza ukukhula kanye nentuthuko yezitshalo.
Ucwaningo ngamaprotheni e-DELLA luqala emuva ku-Green Revolution, lapho ososayensi bengazi besebenzisa amandla abo ukudala izinhlobo ezikhiqiza kakhulu ze-semi-dwarf. Nakuba imininingwane yomsebenzi wabo yayingacacile ngaleso sikhathi, ubuchwepheshe besimanje buvumela ososayensi ukuthi balawule imisebenzi yala maprotheni ngobunjiniyela bezakhi zofuzo, bandise ngempumelelo isivuno sezitshalo.
Isikhathi sokuthunyelwe: Okthoba-23-2025