Abacwaningi boMnyango we-Biochemistry e-Indian Institute of Sciences (IISc) bathole umshini osekunesikhathi eside bewufunaukuze kulawulwe ukukhula kwezitshalo zasendulo ezifana nama-bryophyte (iqembu elihlanganisa ubulembu nama-liverworts) obugcinwe ezitshalweni eziqhakaza kamuva.
Ucwaningo, olushicilelwe kumagazini i-Nature Chemical Biology, lugxile ekulawuleni okungeyona i-canonical yamaprotheni e-DELLA, isilawuli sokukhula esiyinhloko esicindezela ukuhlukana kwamaseli kuma-embryophytes (izitshalo zasemhlabeni).
Kuyathakazelisa ukuthi ama-bryophyte, izitshalo zokuqala ezavela emhlabeni cishe eminyakeni eyizigidi ezingu-500 edlule, ayinayo i-GID1 receptor, naphezu kokukhiqiza i-phytohormone GA. Lokhu kuphakamisa umbuzo wokuthi ukukhula nokuthuthukiswa kwalezi zitshalo zasensimini zakuqala kulawulwa kanjani.
Besebenzisa i-liverwort Marchantia polymorpha njengohlelo lwemodeli, abacwaningi bathola ukuthi lezi zitshalo zasendulo zisebenzisa i-enzyme ekhethekile, i-MpVIH, ekhiqiza isithunywa seselula inositol pyrophosphate (InsP₈), ukudiliza i-DELLA ngaphandle kokubandakanyekai-gibberellik acid.
Abacwaningi basebenzise uhlelo lwe-CRISPR-Cas9 ukuze bakhiphe isakhi sofuzo esifaka ikhodi ye-enzyme ye-VIH, eqinisekisa indima yayo. Izitshalo ezingenayo i-VIH esebenzayo zibonise ukukhubazeka okukhulu kwentuthuko kanye nokungahambi kahle kwe-morphological, njengamaqabunga ahlangene, ukukhula kwe-radial okungasebenzi, nokuntuleka kwama-calyxes. Lezi ziphambeko zaqedwa ngokushintsha i-genome yesitshalo ukuze ikhiqize isiphetho esisodwa kuphela (i-N-terminus) ye-enzyme ye-VIH. Lisebenzisa amasu e-chromatography athuthukisiwe, ithimba lithole ukuthi i-N-terminus iqukethe isizinda se-kinase esenza ukukhiqizwa kwe-InsP₈.
Abacwaningi bathole ukuthi i-DELLA ingenye yezinkomba zamaselula ze-VIH kinase. Ngaphezu kwalokho, baqaphela ukuthi ama-phenotypes ezitshalo ezintula i-MpVIH ayefana nalawo ezitshalo ze-M. polymorpha ezinenkulumo eyandayo ye-DELLA.
"Okwamanje, sikujabulele ukuqonda ukuthi ukuzinza kwe-DELLA noma umsebenzi uyathuthukiswa yini ezitshalweni ezintula i-MpVIH," kusho u-Priyanshi Rana, umbhali wokuqala kanye nomfundi oneziqu eqenjini locwaningo likaLahey. Ngokuvumelana ne-hypothesis yabo, abacwaningi bathola ukuthi ukuvimbela kwe-DELLA kungabuyisela kakhulu ukukhula okunephutha kanye ne-phenotypes yentuthuko yezitshalo eziguquguqukayo ze-MpVIH. Le miphumela iphakamisa ukuthi i-VIH kinase ilawula kabi i-DELLA, ikhuthaza ukukhula nokuthuthuka kwezitshalo.
Ucwaningo lwamaprotheni e-DELLA luhlehlela emuva ku-Green Revolution, lapho ososayensi bengazi besebenzisa amandla abo ukuze bakhe izinhlobo ezikhiqiza kakhulu ze-semi-dwarf. Nakuba imininingwane yomsebenzi wabo yayingacacile ngaleso sikhathi, ubuchwepheshe besimanje buvumela ososayensi ukuba balawule imisebenzi yala maprotheni ngokusebenzisa izakhi zofuzo, bakhulise ngempumelelo isivuno sezitshalo.
Isikhathi sokuthumela: Oct-23-2025