Ukuvela kwesikhashana kokumelana nezibulala-zinambuzane kanye nebhayoloji yezifo ezinkulu zikamalaleveva, omiyane be-Anopheles, e-Uganda

Iyakhulaisibulala-zinambuzaneukumelana kunciphisa ukusebenza kokulawulwa kwe-vector. Ukuqapha ukumelana ne-vector kubalulekile ukuze uqonde ukuvela kwayo kanye nokuklama izimpendulo ezisebenzayo. Kulolu cwaningo, siqaphe amaphethini okumelana nezibulala-zinambuzane, i-vector population biology, kanye nokuhlukahluka kofuzo okuhlobene nokungazweli e-Uganda esikhathini esiyiminyaka emithathu kusukela ngo-2021 kuya ku-2023. E-Mayuga, i-Anopheles funestus ss yayiwuhlobo oluhamba phambili, kodwa kwakukhona ubufakazi bokuhlanganiswa nezinye i-An. izinhlobo ze-funestus. Ukuhlaselwa kwe-sporozoite kwakuphakeme kakhulu, kwafinyelela ku-20.41% ngo-March 2022. Ukumelana okuqinile kwabonwa kuma-pyrethroids izikhathi ezingu-10 zokuhlushwa kokuxilonga, kodwa ukuba sengozini kwatholakala kancane ekuhlolweni kwe-PBO synergy.
Imephu yezindawo zokuqoqa omiyane esifundeni saseMayuge. Isifunda saseMayuge siboniswe ngombala onsundu. Imizana lapho amaqoqo enziwa khona amakwe ngezinkanyezi eziluhlaza. Le mephu yakhiwe kusetshenziswa isofthiwe yomthombo wamahhala nevulekile ye-QGIS version 3.38.
Bonke omiyane babenakekelwa ngaphansi kwezimo ezijwayelekile zokukhuliswa komiyane: 24-28 °C, 65-85% umswakama ohlobene, kanye nenkathi yemvelo engu-12:12 emini. Izibungu zomiyane zakhuliswa emathileyi wezibungu futhi zaphakelwa i-tetramine ad libitum. Amanzi ezibungu ayeshintshwa njalo ezinsukwini ezintathu kuze kube yilapho i-pupat. Abantu abadala abasafufusa babenakekelwa kumakheji e-Bugdom futhi baphakelwa isixazululo sikashukela esingu-10% izinsuku ezingu-3–5 ngaphambi kokuhlolwa kwe-bioassay.
Ukufa ku-pyrethroid bioassay esigabeni se-F1. Ukufa kwama-Spot of Anopheles omiyane kuchayeka kuma-pyrethroids kuphela kanye nama-pyrethroids kuhlanganiswe nama-synergists. Amabha amaphutha kubha namashadi ekholomu amelela izikhawu zokuzethemba ezisekelwe ephutheni elijwayelekile le-mean (SEM), futhi i-NA ibonisa ukuthi uhlolo alwenziwanga. Ulayini ovundlile onamachashazi abomvu umele izinga lokufa elingu-90% elingaphansi lapho ukumelana kuqinisekiswa khona.
Wonke amasethi edatha akhiqizwe noma ahlaziywa phakathi nalolu cwaningo afakiwe esihlokweni esishicilelwe kanye namafayela ayo Olwazi Olwengeziwe.
Inguqulo yokuqala ye-inthanethi yalesi sihloko ishintshiwe: Inguqulo yokuqala yalesi sihloko ishicilelwe ngephutha ngaphansi kwelayisensi ye-CC BY-NC-ND. Ilayisensi isilungisiwe yaba yi-CC BY.