I-Uniconazoleiyi-triazoleumlawuli wokukhula kwezitshalolokho kusetshenziswa kabanzi ukulawula ukuphakama kwesitshalo nokuvimbela ukukhula ngokweqile kwezithombo. Kodwa-ke, indlela yama-molecule i-uniconazole evimbela ngayo ukunwetshwa kwe-hypocotyl yezithombo ayikacaci, futhi kunezifundo ezimbalwa kuphela ezihlanganisa idatha ye-transcriptome ne-metabolome ukuphenya indlela yokunwetshwa kwe-hypocotyl. Lapha, sibonile ukuthi i-uniconazole ivimbele kakhulu ukunwetshwa kwe-hypocotyl ezithombo zeklabishi eziqhakaza zaseShayina. Ngokuthakazelisayo, ngokusekelwe ekuhlaziyweni okuhlanganisiwe kwe-transcriptome ne-metabolome, sithole ukuthi i-uniconazole ithinte kakhulu indlela "ye-phenylpropanoid biosynthesis". Kule ndlela, i-gene eyodwa kuphela yomndeni we-enzyme regulatory gene, i-BrPAL4, ehilelekile ku-lignin biosynthesis, yehliswe kakhulu. Ngaphezu kwalokho, ukuhlolwa kwe-yeast one-hybrid kanye ne-two-hybrid kubonise ukuthi i-BrbZIP39 ingabopha ngqo esifundeni se-promoter se-BrPAL4 futhi isebenze ukukopishwa kwayo. Uhlelo lokuthulisa izakhi zofuzo olubangelwa yigciwane luphinde lwafakazela ukuthi i-BrbZIP39 ingalawula kahle ukunwetshwa kweklabishi yaseShayina kanye nokwakheka kwe-hypocotyl lignin. Imiphumela yalolu cwaningo inikeza ukuqonda okusha ngendlela yokulawula ama-molecule e-cloconazole ekuvimbeleni ukunwetshwa kweklabishi yaseShayina kwe-hypocotyl. Kwaqinisekiswa okokuqala ukuthi i-cloconazole yehlisa okuqukethwe kwe-lignin ngokuvimbela ukwakheka kwe-phenylpropanoid okuqondiswa yi-module ye-BrbZIP39-BrPAL4, ngaleyo ndlela kuholele ekunwetshweni kwe-hypocotyl ezitshalweni zeklabishi yaseShayina.
Iklabishi laseShayina (iBrassica campestris L. ssp. chinensis var. utilis Tsen et Lee) lingelohlobo lweBrassica futhi liyimifino eyaziwa kakhulu yonyaka ekhuliswa kakhulu ezweni lakithi (Wang et al., 2022; Yue et al., 2022). Eminyakeni yamuva nje, izinga lokukhiqizwa kwe-cauliflower yaseShayina liqhubekile nokukhula, futhi indlela yokulima ishintshile kusukela ekuhlwanyeleni okuqondile kwendabuko kuya ekukhuleni kwezithombo ezijulile kanye nokufakelwa. Kodwa-ke, enkambisweni yokukhuliswa kwezithombo ezijulile kanye nokufakelwa, ukukhula kwe-hypocotyl ngokweqile kuvame ukukhiqiza izithombo ezinde, okuholela ekhwalithini embi yezithombo. Ngakho-ke, ukulawula ukukhula kwe-hypocotyl ngokweqile kuyinkinga ebalulekile ekukhuleni kwezithombo ezijulile kanye nokufakelwa kweklabishi laseShayina. Njengamanje, kunezifundo ezimbalwa ezihlanganisa idatha ye-transcriptomics kanye ne-metabolomics ukuhlola indlela yokwandiswa kwe-hypocotyl. Indlela yama-molecule i-chlorantazole elawula ngayo ukwanda kwe-hypocotyl kuklabishi laseShayina ayikafundwa. Sihlose ukuthola ukuthi yiziphi izakhi zofuzo kanye nezindlela zama-molecule ezisabela ku-hypocotyl dwarfing ebangelwa yi-uniconazole eklabishini laseShayina. Sisebenzisa ukuhlaziywa kwe-transcriptome kanye ne-metabolomic, kanye nokuhlaziywa kwe-yeast one-hybrid, ukuhlolwa kwe-dual luciferase, kanye nokuhlolwa kwe-gene silencing (VIGS) okubangelwa yigciwane, sithole ukuthi i-uniconazole ingadala i-hypocotyl dwarfing eklabishini laseShayina ngokuvimbela i-lignin biosynthesis ezitshalweni zeklabishi laseShayina. Imiphumela yethu inikeza ukuqonda okusha ngendlela yokulawula ama-molecule lapho i-uniconazole ivimbela khona ukunwebeka kwe-hypocotyl eklabishini laseShayina ngokuvimbela i-phenylpropanoid biosynthesis eqondiswa yi-BrbZIP39–BrPAL4 module. Le miphumela ingaba nemiphumela ebalulekile esebenzayo yokuthuthukisa ikhwalithi yezithombo zezentengiselwano kanye nokufaka isandla ekuqinisekiseni isivuno kanye nekhwalithi yemifino.
I-BrbZIP39 ORF egcwele ubude yafakwa ku-pGreenll 62-SK ukuze kukhiqizwe i-effector, kwathi i-BrPAL4 promoter fragment yahlanganiswa ne-pGreenll 0800 luciferase (LUC) reporter gene ukuze kukhiqizwe i-reporter gene. Ama-effector kanye nama-reporter gene vectors aguqulwa ndawonye abe amaqabunga kagwayi (i-Nicotiana benthamiana).
Ukuze sicacise ubudlelwano phakathi kwama-metabolites nezakhi zofuzo, senze ukuhlaziywa kwe-metabolome kanye ne-transcriptome ehlangene. Ukuhlaziywa kokunotha kwendlela ye-KEGG kubonise ukuthi ama-DEG nama-DAM ahlanganiswe ndawonye ezindleleni ezingu-33 ze-KEGG (Isithombe 5A). Phakathi kwazo, indlela ye-"phenylpropanoid biosynthesis" yayiyiyona ecebile kakhulu; indlela ye-"photosynthetic carbon fixation", indlela ye-"flavonoid biosynthesis", indlela ye-"pentose-glucuronic acid interconversion", indlela ye-"tryptophan metabolism", kanye nendlela ye-"starch-sucrose metabolism" nazo zicebile kakhulu. Imephu yokuqoqa ukushisa (Isithombe 5B) ibonise ukuthi ama-DAM ahlotshaniswa nama-DEG ahlukaniswe ngezigaba eziningana, phakathi kwazo ama-flavonoid ayeyisigaba esikhulu kunazo zonke, okubonisa ukuthi indlela ye-"phenylpropanoid biosynthesis" idlale indima ebalulekile ku-hypocotyl dwarfism.
Abalobi bathi ucwaningo lwenziwe ngaphandle kobudlelwano bezentengiselwano noma bezezimali obungabhekwa njengokungqubuzana kwezintshisekelo okungenzeka.
Yonke imibono evezwe kulesi sihloko ingeyombhali kuphela futhi ayibonisi ngempela imibono yezinhlangano ezixhumene nayo, abashicileli, abahleli, noma ababuyekezi. Noma yimiphi imikhiqizo ehlolwe kulesi sihloko noma izimangalo ezenziwe abakhiqizi bayo aziqinisekiswa noma azivunyelwe ngumshicileli.
Isikhathi sokuthunyelwe: Mashi-24-2025