Uniconazolei-triazoleisilawuli sokukhula kwezitshaloesetshenziswa kakhulu ukulawula ubude bezitshalo nokuvimbela ukukhula kwezithombo. Kodwa-ke, indlela yamangqamuzana i-uniconazole evimbela ngayo ukunwetshwa kwe-hypocotyl yesithombo ayikacaci, futhi kunezifundo ezimbalwa kuphela ezihlanganisa idatha ye-transcriptome ne-metabolome ukuze kuphenywe indlela yokwelulwa kwe-hypocotyl. Lapha, siqaphele ukuthi i-uniconazole ivimbela kakhulu ukukhula kwe-hypocotyl ezitshalweni zeklabishi eziqhakazayo zaseShayina. Ngokuthakazelisayo, ngokusekelwe ekuhlaziyweni okuhlanganisiwe kwe-transcriptome kanye ne-metabolome, sithole ukuthi i-uniconazole ithinte kakhulu indlela "ye-phenylpropanoid biosynthesis". Kule ndlela, isakhi sofuzo esilawula ama-enzyme, i-BrPAL4, esibandakanyeka ku-lignin biosynthesis, sancishiswa kakhulu. Ngaphezu kwalokho, i-yeast One-hybrid kanye ne-two-hybrid assay ibonise ukuthi i-BrbZIP39 ingabophezela ngokuqondile endaweni yomgqugquzeli we-BRPAL4 futhi yenze kusebenze ukulotshwa kwayo. Uhlelo lokuthulisa izakhi zofuzo olubangelwa yigciwane luphinde lwafakazela ukuthi i-BrbZIP39 ingalawula kahle ukwelulwa kweklabishi laseShayina kanye ne-hypocotyl lignin synthesis. Imiphumela yalolu cwaningo ihlinzeka ngemininingwane emisha endleleni yokulawula yamangqamuzana e-cloconazole ekuvimbeleni ukwelulwa kwe-hypocotyl yeklabishi laseShayina. Kwaqinisekiswa ngokokuqala ngqa ukuthi i-cloconazole yehlisa i-lignin ngokuvimbela ukuhlanganiswa kwe-phenylpropanoid okulamulwa yi-module ye-BrbZIP39-BrPAL4, ngaleyo ndlela kuholele ekuncipheni kwe-hypocotyl ezitshalweni zeklabishi laseShayina.
Iklabishi laseShayina (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) liyingxenye yohlobo lwe-Brassica futhi liyimifino eyaziwa minyaka yonke ye-cruciferous etshalwa kakhulu ezweni lami (Wang et al., 2022; Yue et al., 2022). Eminyakeni yamuva nje, isikali sokukhiqiza ukholifulawa waseShayina siqhubekile nokukhula, futhi indlela yokulima isishintshile kusukela ekutshalweni okuqondile kwendabuko kuya kusiko lokutshala izithombo kanye nokufakelwa kabusha. Kodwa-ke, enqubweni yesiko lokutshala izithombo kanye nokufakelwa kabusha, ukukhula ngokweqile kwe-hypocotyl kuvame ukukhiqiza izithombo ezimile, okuholela emazingeni aphansi wezithombo. Ngakho-ke, ukulawula ukukhula kwe-hypocotyl ngokweqile kuyindaba ecindezelayo esikweni lokutshala izithombo kanye nokufakelwa kabusha kweklabishi laseShayina. Njengamanje, kunezifundo ezimbalwa ezihlanganisa idatha ye-transcriptomics kanye ne-metabolomics ukuhlola indlela yokwelulwa kwe-hypocotyl. Indlela yamangqamuzana i-chlorantazole elawula ngayo ukwanda kwe-hypocotyl eklabishini laseShayina ayikahlolisiswa. Sasihlose ukuhlonza ukuthi yiziphi izakhi zofuzo nezindlela zamangqamuzana ezisabela ekuncipheni kwe-hypocotyl eyenziwe nge-uniconazole kuklabishi lase-Chinese. Sisebenzisa ukuhlaziywa kwe-transcriptome kanye ne-metabolomic, kanye nokuhlaziywa kweyeast one-hybrid, i-dual luciferase assay, nokuhlolwa kofuzo olubangelwa yigciwane (VIGS), sithole ukuthi i-uniconazole ingenza i-hypocotyl dwarfing eklabishini laseShayina ngokuvimbela i-lignin biosynthesis kusithombo seklabishi lesiShayina. Imiphumela yethu ihlinzeka ngemininingwane emisha endleleni yokulawula yamangqamuzana lapho i-uniconazole ivimbela ukwelulwa kwe-hypocotyl eklabishini laseShayina ngokuvimbela i-phenylpropanoid biosynthesis elamula imojuli ye-BrbZIP39–BrPAL4. Le miphumela ingase ibe nemithelela ebalulekile ekwenzeni ngcono izinga lezithombo ezithengiswayo futhi ibe negalelo ekuqinisekiseni isivuno kanye nekhwalithi yemifino.
I-BrbZIP39 ORF yobude obugcwele yafakwa ku-pGreenll 62-SK ukuze kukhiqizwe umthelela, futhi ucezu lomthuthukisi we-BRPAL4 lwahlanganiswa nofuzo lwentatheli ye-pGreenll 0800 luciferase (LUC) ukuze kukhiqizwe ufuzo lwentatheli. I-effector ne-reporter gene vectors yaguqulwa yaba amahlamvu kagwayi (Nicotiana benthamiana).
Ukucacisa ubudlelwano bama-metabolites nezakhi zofuzo, senze i-metabolome ehlanganyelwe kanye nokuhlaziywa kwe-transcriptome. Ukuhlaziywa kwendlela ye-KEGG yokunothisa kubonise ukuthi ama-DEG nama-DAM acetshiswe ngokubambisana emigwaqweni engama-33 ye-KEGG (Umfanekiso 5A). Phakathi kwabo, indlela "phenylpropanoid biosynthesis" yayicetshiswe kakhulu; indlela "ye-photosynthetic carbon fixation", indlela "ye-flavonoid biosynthesis", indlela "ye-pentose-glucuronic acid interconversion", indlela "ye-tryptophan metabolism", kanye nendlela "ye-starch-sucrose metabolism" nazo zathuthukiswa kakhulu. Imephu yokuhlanganisa ukushisa (Umfanekiso 5B) ibonise ukuthi ama-DAM ahlotshaniswa nama-DEG ahlukaniswe abe izigaba eziningana, phakathi kwazo ama-flavonoid ayeyisigaba esikhulu kunazo zonke, okubonisa ukuthi indlela "ye-phenylpropanoid biosynthesis" idlale indima ebalulekile ku-hypocotyl dwarfism.
Ababhali bamemezela ukuthi ucwaningo lwenziwe ngaphandle kobudlelwane bezentengiselwano noma bezezimali obungahunyushwa njengokushayisana kwezintshisekelo okungenzeka.
Yonke imibono evezwe kulesi sihloko ngeyombhali kuphela futhi ayibonisi imibono yezinhlangano ezixhumene, abashicileli, abahleli, noma ababuyekezi. Noma yimiphi imikhiqizo ehlolwe kulesi sihloko noma izimangalo ezenziwe abakhiqizi bazo aziqinisekisiwe noma zigunyazwe umshicileli.
Isikhathi sokuthumela: Mar-24-2025