Njengenye yezinkinga ezinkulu ze-abiotic, ukucindezeleka okuphansi kwezinga lokushisa kuphazamisa kakhulu ukukhula kwezitshalo futhi kuthinta kabi isivuno kanye nekhwalithi yezitshalo. I-5-Aminolevulinic acid (ALA) ilawula ukukhula etholakala kakhulu ezilwaneni nasezitshalweni. Ngenxa yokusebenza kwayo kahle, ukungabi nobuthi kanye nokuwohloka kalula, isetshenziswa kabanzi enkambisweni yokubekezelela ubanda kwezitshalo.
Kodwa-ke, ucwaningo oluningi lwamanje oluhlobene ne-ALA lugxile kakhulu ekulawuleni ama-endpoint enethiwekhi. Indlela ethile yokusebenza kwama-molecule yesenzo se-ALA ekubekezeleleni umkhuhlane kwezitshalo ekuqaleni ayicaci okwamanje futhi idinga ucwaningo olwengeziwe ngososayensi.
NgoJanuwari 2024, i-Horticultural Research yashicilela iphepha locwaningo elinesihloko esithi “I-5-Aminolevulinic Acid Ithuthukisa Ukubekezelelana Kokubanda Ngokulawula i-SlMYB4/SlMYB88-SlGSTU43 Reactive Oxygen Species Scavenging Module kuTomato” yithimba likaHu Xiaohui eNorthwestern University kwezolimo namahlathi.
Kulolu cwaningo, i-glutathione S-transferase gene SlGSTU43 yatholakala kutamatisi (i-Solanum lycopersicum L.). Imiphumela yocwaningo ikhombisile ukuthi i-ALA ibangela kakhulu ukuvezwa kwe-SlGSTU43 ngaphansi kokucindezeleka okubandayo. Imigqa katamatisi eguqulwe ngendlela ye-transgenic eveza ngokweqile i-SlGSTU43 ibonise ukwanda okukhulu kwamandla okususa izinhlobo ze-oxygen ezisabelayo futhi yabonisa ukumelana okukhulu nokucindezeleka okuphansi kokushisa, kanti imigqa eguquliwe ye-SlGSTU43 yayizwela ukucindezeleka okuphansi kokushisa.
Ngaphezu kwalokho, imiphumela yocwaningo ikhombisile ukuthi i-ALA ayikhulisi ukubekezelelana kohlobo lwe-mutant kuya ekucindezelekeni okuphansi kokushisa. Ngakho-ke, ucwaningo lusikisela ukuthi i-SlGSTU43 iyisakhi sofuzo esibalulekile enkambisweni yokuthuthukisa ukubekezelelana kokubanda kutamatisi yi-ALA (Isithombe 1).
Ngaphezu kwalokho, lolu cwaningo luqinisekisile ngokutholwa kwe-EMSA, Y1H, LUC kanye ne-ChIP-qPCR ukuthi i-SlMYB4 kanye ne-SlMYB88 zingalawula ukuvezwa kwe-SlGSTU43 ngokubopha kumgqugquzeli we-SlGSTU43. Ukuhlolwa okwengeziwe kubonise ukuthi i-SlMYB4 kanye ne-SlMYB88 nazo zihilelekile enqubweni ye-ALC ngokwandisa ukubekezelelana kukatamatisi ekucindezelekeni okuphansi kwezinga lokushisa kanye nokulawula kahle ukuvezwa kwe-SlGSTU43 (Isithombe 2). Le miphumela inikeza ukuqonda okusha ngendlela i-ALA ethuthukisa ngayo ukubekezelelana ekucindezelekeni okuphansi kwezinga lokushisa kutamatisi.
Ulwazi olwengeziwe: Zhengda Zhang et al., i-5-aminolevulinic acid ithuthukisa ukubekezelelana kokubanda ngokulawula imodyuli ye-SlMYB4/SlMYB88-SlGSTU43 yokufuna izinhlobo ze-oxygen ezisebenzayo kutamatisi, i-Horticulture Research (2024). I-DOI: 10.1093/hour/uhae026
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Isikhathi sokuthunyelwe: Julayi-22-2024