Ukulawula ukukhula kwe-5-aminolevulinic acid kwandisa ukumelana okubandayo kwezitshalo zamatamatisi.

      Njengenye yezingcindezi ezinkulu ze-abiotic, izinga lokushisa eliphansi livimbela ukukhula kwezitshalo futhi lithinte kabi isivuno kanye nekhwalithi yezitshalo.I-5-Aminolevulinic acid (ALA) iyisilawuli sokukhula esitholakala kakhulu ezilwaneni nasezitshalweni.Ngenxa yokusebenza kwayo okuphezulu, ukungabi nobuthi kanye nokuwohloka kalula, isetshenziswa kabanzi enqubweni yokubekezelelana okubandayo kwezitshalo.
Kodwa-ke, ucwaningo oluningi lwamanje oluhlobene ne-ALA lugxile kakhulu ekulawuleni izindawo zokugcina zenethiwekhi.Indlela ethile yamangqamuzana yesenzo se-ALA ekubekezeleleni amakhaza kwangaphambi kwesikhathi kwezitshalo okwamanje ayicacile futhi idinga ucwaningo olwengeziwe ngososayensi.
NgoJanuwari 2024, i-Horticultural Research yashicilela iphepha locwaningo elinesihloko esithi “5-Aminolevulinic Acid Enhances Cold Tolerance by Regulating the SlMYB4/SlMYB88-SlGSTU43 Reactive Oxygen Species Scavenging Module in Tomato” yithimba likaHu Xiaohui eNyakatho Ntshonalanga yezolimo kanye neNyuvesi yaseNyakatho.
Kulolu cwaningo, i-glutathione S-transferase gene SlGSTU43 ikhonjwe kutamatisi (Solanum lycopersicum L.).Imiphumela yocwaningo ibonise ukuthi i-ALA ikhuthaza kakhulu inkulumo ye-SlGSTU43 ngaphansi kwengcindezi ebandayo.Imigqa ye-transgenic tomato overexpressing SlGSTU43 ibonise ukwanda okukhulu kwezinhlobo zomoya-mpilo osebenzayo futhi ibonise ukumelana okuphawulekayo nokucindezeleka okuphansi kwezinga lokushisa, kuyilapho imigqa eguquguqukayo ye-SlGSTU43 yayizwela ekucindezelekeni okuphansi kwezinga lokushisa.
Ngaphezu kwalokho, imiphumela yocwaningo ibonise ukuthi i-ALA ayikukhulisi ukubekezelelana kohlobo oluguquguqukayo ekucindezelekeni okuphansi kwezinga lokushisa.Ngakho-ke, ucwaningo lubonisa ukuthi i-SlGSTU43 iyisakhi esibalulekile ekuthuthukiseni ukubekezelelana okubandayo kutamatisi nge-ALA (Fig. 1).
Ngaphezu kwalokho, lolu cwaningo luqinisekise ngokutholwa kwe-EMSA, Y1H, LUC kanye ne-ChIP-qPCR ukuthi i-SlMYB4 ne-SlMYB88 ingalawula ukuvezwa kwe-SLGSTU43 ngokubophezela kumgqugquzeli we-SlGSTU43.Ukuhlola okwengeziwe kubonise ukuthi i-SlMYB4 ne-SlMYB88 nazo zihilelekile kunqubo ye-ALC ngokukhulisa ukubekezelela utamatisi ekucindezelekeni okuphansi kwezinga lokushisa nokulawula kahle ukubonakaliswa kwe-SLGSTU43 (Fig. 2).Le miphumela inikeza ukuqonda okusha endleleni i-ALA ethuthukisa ngayo ukubekezelelana nokucindezeleka okuphansi kwezinga lokushisa kutamatisi.
Ulwazi olwengeziwe: U-Zhengda Zhang et al., I-5-aminolevulinic acid ithuthukisa ukubekezelelana okubandayo ngokulawula i-module ye-SlMYB4/SlMYB88-SlGSTU43 yezinhlobo ze-oksijini ezisebenzayo ezisakaza utamatisi, Ucwaningo lwe-Horticulture (2024).I-DOI: 10.1093/ihora/uhae026
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