Yimuphi umehluko phakathi kwe-zeatin, i-Trans-zeatin kanye ne-zeatin riboside? Ziyini izindlela ezisetshenziswa ngazo?

Imisebenzi eyinhloko

1. Khuthaza ukuhlukaniswa kwamaseli, ikakhulukazi ukuhlukaniswa kwe-cytoplasm;

2. Khuthaza ukuhlukaniswa kwamahlumela. Ekukhuleni kwezicubu, isebenzisana ne-auxin ukulawula ukuhlukaniswa nokwakheka kwezimpande namahlumela;

3. Khuthaza ukuthuthukiswa kwamahlumela aseceleni, kuqedwe ukubuswa kwamahlumela asezinhlakeni, ngaleyo ndlela kuholele ekwakhekeni kwenqwaba yamahlumela aqhamukayo ekukhuleni kwezicubu;

4. Ukulibazisa ukuguga kwamaqabunga, ukunciphisa izinga lokuwohloka kwe-chlorophyll namaprotheni;

5. Hlukanisa imbewu ingavuki, shintsha ukukhanya ukuze kuhlangatshezwane nezidingo zokukhanya zembewu efana nogwayi;

6. Faka i-parthenocarpy ezithelweni ezithile;

7. Khuthaza ukwakheka kwama-initials amahlumela: emaphethelweni amaqabunga anqunyiwe kanye nakwezinye izimila, kungakhuthaza ukwakheka kwama-initials amahlumela;

8. Khuthaza ukwakheka kwezimila zamazambane.

Iqukethe kuphela isakhiwo se-trans futhi inomphumela ofanayo nowe-i-zeatin, kodwa ngomsebenzi oqinile.

Umphumela wayo ufana kakhulu nowe-anti-zeatin. Ayigcini nje ngokuba nemisebenzi ye-zeatin eshiwo ngenhla, kodwa futhi inomphumela wokuvuselela ukubonakaliswa kwezakhi zofuzo kanye nomsebenzi we-metabolic.

Indlela Yokusebenzisa

1. Khuthaza ukuhluma kwe-callus (kumele isetshenziswe kanye ne-auxin), ukuhlushwa okungu-1mg/L.

2. Khuthaza ukulungiselelwa kwezithelo, i-1001 mg/L zeatin + 5001 mg/L GA3 + 201 mg/L NAA, fafaza izithelo ezinsukwini eziyi-10, 25, kanye nezingama-40 ngemva kokuqhakaza.

3. Ngemifino enamaqabunga, fafaza ngo-201 mg/L ukuze ubambezele ukuphuzi kwamaqabunga.

Ngaphezu kwalokho, ukwelapha ezinye izimbewu zezitshalo kungakhuthaza ukuhluma; ukwelapha esigabeni sokuhluma kwezithombo kungakhuthaza ukukhula.

1. Khuthaza ukuhluma kwezicubu ze-callus (kumele kusetshenziswe kanye ne-auxin), ekugxilweni kwe-1 ppm;

2. Khuthaza ukulungiselelwa kwezithelo, i-100 ppm ye-cytokinin + 500 ppm ye-GA3 + 20 ppm ye-NAA, fafaza izithelo ezinsukwini eziyi-10, 25, kanye nezingama-40 ngemva kokuqhakaza;

3. Libazisa ukuphuzi kwamaqabunga emifino, fafaza ngo-20 ppm;

1. Ekukhuleni kwezicubu zezitshalo, ukuhlushwa okuvamile kwe-anti-cytokinin nucleoside kungu-1 mg/mL noma ngaphezulu.

2. Ekulawulweni kokukhula kwezitshalo, ukuhlushwa kwe-anti-cytokinin nucleoside kuvame ukuba yi-1 ppm kuya ku-100 ppm, futhi ukuhlushwa okuthile kuncike ekusetshenzisweni okuthile kanye nezinhlobo zezitshalo. Isibonelo, lapho kukhuthazwa ukuhluma kwezicubu ze-callus, ukuhlushwa kwe-anti-cytokinin nucleoside kuyi-1 ppm, futhi kudingeka kusetshenziswe kanye ne-auxin.

3. Ncibilikisa kahle i-anti-cytokinin nucleoside powder nge-2-5 mL ye-1 M NaOH (noma i-1 M acetic acid noma i-1 M KOH), bese ufaka amanzi acwengekile kabili noma amanzi amsulwa kakhulu ukuze ulungiselele isisombululo sokugcina esingu-1 mg/mL noma ngaphezulu. Hlanganisa ngenkathi ufaka amanzi ukuqinisekisa ukuxubana kahle. Isixazululo sokugcina kufanele sihlanganiswe futhi siqandiswe ukuze kugwenywe ukuncibilika okuphindaphindiwe. Ncibilikisa isisombululo sokugcina nge-culture medium kuya ekugxilweni okudingekayo, bese ulungisa isisombululo esisebenzayo khona lapho bese usisebenzisa ngokushesha.

Ekuphetheni, i-zeatin, i-abscisic acid kanye ne-abscisic acid nucleotide ngayinye inezimpawu zayo ngokwesakhiwo, umsebenzi kanye nezicelo zokusebenza. Ekuphetheni, i-zeatin, i-abscisic acid kanye ne-abscisic acid nucleotide ngayinye inezimpawu zayo ngokwesakhiwo, umsebenzi kanye nezicelo zokusebenza. Kodwa-ke, zonke zisebenza njengabalawuli bokukhula kwezitshalo futhi zidlala indima ebalulekile ekukhuthazeni ukukhula kwezitshalo kanye nentuthuko.