Uyini umehluko phakathi kwe-zeatin, i-Trans-zeatin ne-zeatin riboside? Yiziphi izicelo zabo?

Imisebenzi esemqoka

1. Ukuthuthukisa ukuhlukana kwamaseli, ikakhulukazi ukuhlukaniswa kwe-cytoplasm;

2. Thuthukisa ukuhlukaniswa kwehlumela. Esikweni lezicubu, lisebenzisana ne-auxin ukulawula ukuhlukaniswa nokwakheka kwezimpande nama-buds;

3. Ukukhuthaza ukuthuthukiswa kwama-lateral buds, ukuqeda ukubusa kwe-apical, futhi ngaleyo ndlela kuholele ekwakhiweni kwenani elikhulu lama-adventitious buds kusiko lwezicubu;

4. Ukubambezeleka kwamaqabunga, nciphisa izinga lokuwohloka kwe-chlorophyll namaprotheni;

5. Phula imbewu, shintsha ukukhanya ukuze uhlangabezane nemfuneko yokukhanya yembewu enjengogwayi;

6. Faka i-parthenocarpy ezithelweni ezithile;

7. Khuthaza ukwakheka kwama-initials ama-bud: ekugcineni kwamaqabunga nakweminye i-mosses, kungakhuthaza ukwakheka kwama-initials ama-bud;

8. Khuthaza ukwakheka kwezilimo eziyizigaxa zamazambane.

Iqukethe kuphela ukwakheka kwe-trans futhi inomphumela ofanayo nezeatin, kodwa ngomsebenzi onamandla.

Umphumela wayo ufana kakhulu nowe-anti-zeatin. Ayinayo nje imisebenzi eshiwo ngenhla ye-zeatin, kodwa futhi inomphumela wokuvula ukubonakaliswa kofuzo kanye nomsebenzi we-metabolic.

Indlela Yokusebenzisa

1. Khuthaza ukuhluma kwe-callus (kufanele kusetshenziswe kuhlanganiswe ne-auxin), ukugxilisa ingqondo 1mg/L.

2. Thuthukisa ukulungiselelwa kwezithelo, 1001 mg/L zeatin + 5001 mg/L GA3 + 201 mg/L NAA, fafaza esithelweni 10, 25, kanye nezinsuku ezingu-40 ngemva kokuqhakaza.

3. Ngemifino enamahlamvu, fafaza ngo-201 mg/L ukubambezela ukuphuzi kwamaqabunga.

Ngaphezu kwalokho, ukwelapha ezinye izimbewu zezitshalo kungakhuthaza ukuhluma; ukwelapha esigabeni sezithombo kungathuthukisa ukukhula.

1. Khuthaza ukuhluma kwezicubu ze-callus (kufanele kusetshenziswe ngokuhambisana ne-auxin), ekuhlanganiseni kwe-1 ppm;

2. Nyusa ukulungiselelwa kwezithelo, 100 ppm we-cytokinin + 500 ppm we-GA3 + 20 ppm we-NAA, fafaza izithelo ezinsukwini ezingu-10, 25, nezingu-40 ngemva kokuqhakaza;

3. Libazisa ukuphuzi kwamaqabunga emifino, fafaza u-20 ppm;

1. Kusiko lwezicubu zezitshalo, ukuhlushwa okuvamile kwe-anti-cytokinin nucleoside ngu-1 mg/mL noma ngaphezulu.

2. Ekulawulweni kokukhula kwezitshalo, ukugxila kwe-anti-cytokinin nucleoside kuvame ukuba ngu-1 ppm kuya ku-100 ppm, futhi ukugxila okukhethekile kuncike ekusetshenzisweni okukhethekile kanye nezinhlobo zezitshalo. Isibonelo, uma kukhuthazwa ukuhluma kwezicubu ze-callus, ukugxila kwe-anti-cytokinin nucleoside yi-1 ppm, futhi idinga ukusetshenziswa ngokuhambisana ne-auxin.

3. Chaza i-anti-cytokinin nucleoside powder kahle nge-2-5 mL ye-1 M NaOH (noma i-1 M acetic acid noma i-1 M KOH), bese wengeza amanzi ahlanjululwe kabili noma amanzi e-ultrapure ukuze ulungiselele isisombululo sokugcina esingu-1 mg/mL noma ukugxila okuphezulu. Govuza ngenkathi ungeza amanzi ukuze uqinisekise ukuxutshwa okuphelele. Isixazululo sesitoreji kufanele sinqanyulwe futhi siqandiswe ukuze kugwenywe ukuncibilika kweqhwa okuphindaphindiwe. Nciphisa isisombululo sokugcina nge-culture medium kuya ekugxiliseni okudingekayo, futhi ulungise isisombululo sokusebenza ngaso leso sikhathi futhi usisebenzise ngokushesha.

Ekuphetheni, i-zeatin, i-abscisic acid ne-abscisic acid nucleotide ngayinye inezici zayo mayelana nesakhiwo, umsebenzi kanye nezicelo ezisebenzayo. Kodwa-ke, zonke zisebenza njengezilawuli zokukhula kwezitshalo futhi zidlala indima ebalulekile ekukhuthazeni ukukhula nokuthuthuka kwezitshalo.