I-γ-Valactone ikhuthaza ukukhula kwezithombo zikatamatisi ngezinguquko ze-transcriptome kanye nama-hormone ezitshalo | I-Plant Molecular Biology

Ukuze kuncishiswe ukuthembela kwezolimo kumanyolo wamakhemikhali, intshisekelo yezinhlobo ezintsha zomanyolo iyakhula. Amakhemikhali abalulekile ayatholakalaabagqugquzeli bokukhula abathembisayo.I-Gamma-valactone, i-compound eguquguqukayo ekhiqizwa yi-*S. stearothermiae* NRCB010, iboniswe ukuthi ikhuthaza ukukhula kwezithombo zikatamatisi ngaphansi kwezimo ezihlanzekile. Ngakho-ke, sihlole imiphumela yokugxila okuhlukahlukene kwe-gamma-valactone ekukhuleni kwezithombo zikatamatisi ngaphansi kwezimo ze-hydroponic kanye ne-greenhouse, futhi sihlole nezinguquko ezifanayo emazingeni okubhalwa kanye nama-hormone. Imiphumela ibonise ukuthi ukwelashwa kwe-gamma-valactone kukhuthaze kakhulu ukukhula kwezithombo zikatamatisi, ngokugxila okuhle kakhulu okungu-100 mg/L kanye no-250 mg/L ngaphansi kwezimo ze-hydroponic kanye ne-greenhouse, ngokulandelana. Okuqukethwe yi-IAA-aspartic acid, i-IAA-glutamic acid,i-indole-3-acetamide, i-indole-3-acetonitrile, i-indole-3-butyric acid, kanye ne-gibberellin emaqabunga, iziqu, kanye nezimpande kwanda kakhulu. Ukuhlaziywa kwe-Transcriptome kwembule ukuthi ngemva kwamahora ayi-6 okwelashwa kwe-γ-valerolactone, inani lezakhi zofuzo ezivezwe ngokuhlukile (ama-DEG) anokubonakaliswa okwandisiwe lidlule inani lezakhi zofuzo ezinokubonakaliswa okwehla; noma kunjalo, isimo esiphambene sabonwa ngemva kwamahora angama-24 okwelashwa. I-γ-valerolactone ingakhuthaza ukukhula kwezithombo zikatamatisi ngezindlela ezifana ne-photosynthesis, i-plant hormone signaling, kanye ne-metabolite biosynthesis yesibili. Lolu cwaningo luhlinzeka ngesisekelo semfundiso yokusetshenziswa kwe-γ-valerolactone njengomlawuli omusha wokukhula kwezitshalo futhi lubeka isisekelo sokuthuthukiswa kwe-biostimulants yezolimo esebenza kahle kakhulu futhi enobungani nemvelo.

Ukuhlaziywa kwesimo somzimba, i-transcriptomic kanye ne-hormone kubonise ukuthi i-γ-valerolactone ithuthukisa ukukhula kwezithombo zikatamatisi futhi inyusa amazinga ama-hormone.
UBasu A, uPrasad P, uDas SN, uKalam S, uSyed RZ, uReddy MS, u-El Enshasy H (2021) Amagciwane e-rhizosphere akhuthaza ukukhula kwezitshalo (PGPR) njengezinto eziphilayo ezinobungani nemvelo: intuthuko yakamuva, imikhawulo, kanye namathemba. Ukusimama 13(3): 1140
I-Campobenedetto S, i-Mannino G, i-Bickwilder J, i-Contartesse V, i-Karlova R, i-Bertea CM (2021) Ukusetshenziswa kwe-biostimulants esekelwe ku-tannin kuthinta isakhiwo sezimpande zikatamatisi futhi kuthuthukisa ukubekezelelana kukasawoti. I-Sci Rep 11(1):354
I-Cao Hong, i-Gong Hong, i-Song Tao, i-Yu Ming, i-Pan Xin, i-Yu Juan, i-Qi Zhi, i-Du Yan, i-Liu Yan (2022) Iphrotheni ye-adaptha i-UvSte50 ilawula i-pathogenicity ye-*Ustilago maydis* ngokusebenzisa indlela yokusayina ye-MAPK. *Acta Mycologica Sinica* 8(9):954
UCastiglione AM, Mannino G., Contartesse V., Bertea CM, Ertani A. (2021) Ama-biostimulants e-Microbial ukuhlangabezana nezidingo zezolimo zanamuhla: ukwakheka, imisebenzi, kanye nokusetshenziswa kwale mikhiqizo emisha. I-Plants-Basel 10(8):1533
UChandel SS, Sharma KD (2023) Ukwehla kwezakhi zofuzo zendlela yokuguqulwa kwama-carbohydrate ngaphansi kokucindezeleka kokushisa okuphezulu kuholele ekunciphiseni okuqukethwe kwe-sucrose kanye nesitashi emaqabunga e-chickpea. Izinqubo ze-Indian Academy of Sciences 46(5):445–449
UChen K, uLi GJ, uBressan RA, uSong CP, uZhu JQ, uZhao Y (2020a) Amandla, isignali, kanye nomsebenzi we-abscisic acid ezitshalweni. J Integr Plant Biol 62(1):25–54
UChen S, uZhao Y, uZhao H, uChen S (2020b) Ukuhlonza izakhi zofuzo ze-lignin biosynthetic ezifakazelwa ku-birch. Isakhiwo Sezinkuni Nomsebenzi 34(5):1255–1265
UDing Z, Yao Y, Yao K, Hou H, Zhang Z, Huang Y, Wang S, Liao W (2024) SlSERK3B ikhuthaza ukukhula nokuthuthukiswa kwezithombo zikatamatisi ngokulawula amandla e-photosynthetic. Ijenali Yamazwe Ngamazwe Yesayensi Yamangqamuzana 25(2):1336
U-Geng Peng, u-Zhang Shuai, u-Liu Jie, u-Zhao Chao, u-Wu Jun, u-Cao Yong, u-Fu Chao, u-Han Xin, u-He Hui, u-Zhao Qiang (2020) MYB20, MYB42, MYB43, kanye ne-MYB85 balawula i-phenylalanine kanye ne-lignin biosynthesis ngesikhathi sokwakhiwa kweseli yesibili] I-Acta Physiologica Sinica 182(3):1272–1283
UGuo Z, uChen Y, uWu D, uDu Y, uWang M, uLiu Z, uChu J, uYao S (2022) Ukuhlaziywa kwe-Transcriptome kwembula indima ebalulekile ye-brassinolide engaphandle endleleni ye-alkaloid biosynthetic yePinellia ternata. I-International Journal of Molecular Sciences 23(18):10898
UGupta K, Wani SH, Razak A, Skalicki M, Samantara K, Gupta S, Pandita D, Goel S, Grewal S, Heynak V, Shiv A, El-Sabrout AM, Elansari HO, Alaklabi A, Brestik M (2022) I-Abscisic acid: indima yayo ekuthuthukisweni nasekuvuthweni kwezithelo. Front Plant Sci 13:817500
UHildebrandt TM, uNesi AN, u-Araujo VL, uBrown HP (2015) Ukushintshwa kwe-amino acid ezitshalweni. Isitshalo seMol 8(11):1563–1579
UHorvath IT, Mehdi H, Fabos V, Boda L, Mika LT (2008) i-γ-velolactone: amandla ahlala isikhathi eside kanye noketshezi lwamakhemikhali oluqukethe ikhabhoni. I-Green Chem 10(2):238–242
Khalid F, Rashid Y, Asif K, Ashraf H, Maqsood MF, Shahbaz M, Zulfiqar U, Sardar R, Haider FW (2024) Izinto ezivuselela izitshalo: izindlela kanye nezicelo zokuthuthukisa ukuzivumelanisa nezitshalo nokucindezeleka okungenabungozi. Ijenali Yesayensi Yenhlabathi Nokudla Kwezitshalo 24:6641–6690

Kishore PBK, Tiozone RN Jr, Fernie AR, Srinivasulu N (2022) I-Abscisic acid kanye nendima yayo ekulawuleni ukukhula kwezitshalo, intuthuko, kanye nokuqina kwesivuno. Amathrendi kuSayensi Yezitshalo 27(12):1283–1295
UKumar K, uRaina SK, uSultan SM (2020) Indlela yokubonisana ye-Arabidopsis MAPK kanye nokusebenzisana kwayo ekuphenduleni ukucindezeleka okungenabungozi. Ijenali ye-Plant Biochemistry kanye ne-Biotechnology 29(4):700–714
ULee D, uYeon YJ (2020) Ukuhlanganiswa kwe-(R)-γ-valerate kusuka ku-levulinic acid ngendlela ye-enantioselective chemoenzymatic. Inqubo ye-Biochem 90:113–117
ULi F, uWu C, uLiao B, uYu K, uHo Y, uMeng L, uWang S, uWang B, uDu M, uTian X, uLi Z (2022) I-Thiadizoline ikhuthaza ukuqunjelwa kwamaqabunga ekotini ngokulawula ukusebenzisana okuyinkimbinkimbi phakathi kwe-ethylene, i-auxin, ne-cytokinin. I-International Journal of Molecular Sciences 23(5):2696
ULi J, uMin S, uLuo Q, u-Abdoulaye AH, uZhang S, uHuang W, uZhang R, uChen Y (2023a) Ukuhlukaniswa kwama-molecule omndeni we-GH3 ku-alfalfa ngaphansi kokucindezeleka okungenabungozi. I-Gene 851:146982
ULi Yan, uHan Shuai, uQi Yong (2023b) Inqubekela phambili yocwaningo ngesakhiwo kanye nemisebenzi yezinto eziphendula i-plant auxin. Ijenali ye-Integrative Plant Biology 65(3):617–632
Ma X, Zhang Y, Tureckova V, Xue GP, Ferney AR, Müller-Robert B, Balazadeh S (2018) I-NAC transcription factor SlNAP2 ilawula ukuvela kweqabunga likatamatisi kanye nesivuno sezithelo. I-Acta Physiologica Sinica 177(3):1286–1302.
UMachik M., uGryta A., uFrack M. (2020) Izivundisi ze-bio zezolimo: imiqondo, amasu, kanye nomthelela ezilwanyaneni zenhlabathi. Intuthuko Kwezolimo 162:31–87
UMoshinets OV, uBabenko LM, uRogalsky SP, uJungin OS, uFoster J, uKosakiwska IV, uPotters G, uSpears AJ (2019) babonise amandla okuthuthukisa ukukhula kwezitshalo kanye nokuvunwa kwembewu ngokwelapha imbewu kakolweni yasebusika kusengaphambili ngesignali ye-bacterium quorum-sensing N-hexanoyl-L-homoserine lactone (C6-HSL). PLoS ONE 14(2):e0209460
UMüller M. (2021) Isitha nomngane akuhlukaniseki: ukusebenzisana kwe-abscisic acid ne-ethylene ngaphansi kokucindezeleka okungenabungozi. Izitshalo-Basel 10(3):448
UMoon HI, uKwon MK, uLee NR, uSong SY, uSong DH, uLee CH (2021) baqhathanise ama-metabolites kanye nezakhiwo zokusebenza zamatamatisi ahlukene besebenzisa i-metabolomics esekelwe ku-mass spectrometry. Front Nutr 8:659646
UNing Yi, uDing Yongkang, uChang Yonghong, uZhang Shuai, u-An Haiming, uFu Yongjun (2023) Amazinga aphansi e-methyl jasmonate akhuthaza ukukhula kwe-Podocarpus macrophyllus ngokulinganisela phakathi kokubonisana kwama-hormone ezitshalo kanye nokwakheka kwe-triterpenoid. Umthetho Wokukhula Kwezitshalo 101(1):187–199
Pavlovich PV, Koshy P (2022) Umehluko ekulandeleni kokubonakaliswa kwezakhi zofuzo: ukuhlaziywa kwedatha ye-RNA-seq. Izindlela i-Mol Biol (Clifton NJ) 2508:279–318
UPrisa D, Fresco R, Spagnuolo D (2023) Ukusetshenziswa kwezivundisi ze-microbial ekukhiqizweni kwezitshalo kanye nokuthuthukiswa kokumelana: isibuyekezo. Agric Basel 13(9):1666
URen Feng, uLiu Ning, uGao Bing, uShen Hui, uLi Shuai, uLi Li, uZheng Dan, uShen Wei, uGao Ning (2024) Ukuhlonza amakhemikhali e-volatile organic ku-*Stenotrophomonas stearothermiae* ukuze kuthuthukiswe ukwakheka kwamakoloni nokukhuthaza ukukhula kwezithombo zikatamatisi. Ijenali ye-Applied Microbiology 135(10):lxae248
U-Ullah S, uSchmidt A, uReichelt M, uTsai SJ, uGershenzon J (2022) Akukho ukusebenzisana okuphikisayo okukhona phakathi kwezindlela zokubonisa i-salicylic ne-jasmonic acid ku-poplar. I-New Botanist 235(2):701–717
UWang Zhiqiang, uGou Xiaodong (2020) Ama-protein kinase afana nama-receptor atholakala phezulu kwama-MAPK ekulawulweni kwentuthuko yezitshalo. I-International Journal of Molecular Sciences 21(20):7638
UWang Chao, uZhang Jie, uLi Jie, uXie Jun, uChai Qiang (2024) I-exogenous methyl jasmonate ilawula ukwakheka kwama-hormone e-endogenous kuma-leek akhuliswe ngamanzi ukuze kukhuthazwe ukukhula kwawo kanye ne-photosynthesis. Isayensi Yezolimo 327:112861
UYoung T, uDavis PJ, uReid JB (1996) Ukuhlaziywa kwezakhi zofuzo kwezindima ezihlobene ze-auxin ne-gibberellin ekulawulweni kokwandiswa kwesiqu se-pea ngaphansi kokukhanya okugcwele. Acta Physiologica Sinica 110(3):1029–1034
Yu Z, Zhang F, Friml J, Ding Z (2022) I-Auxin signaling: inqubekela phambili yocwaningo eminyakeni engama-30 edlule. J Integr Plant Biol 64(2):371–392

U-Zhang Hua, u-Zheng Dan, u-Hu Chao, u-Cheng Wei, u-Lei Peng, u-Xu Hui, u-Gao Ning (2023) ama-metabolites e-Pseudomonas NRCB010 enza ama-exudate ezimpande ezithile zikatamatisi ukuze kuthuthukiswe ikoloni ye-rhizosphere. 13(5):664
UZhou Chao, uLi Shuai, uZheng Yong, uLei Peng, uChen Yong, uYing Hua, uGao Ning (2022) Inhlanganisela ye-phosphate enamandla aphezulu ikhuthaza ukukhula kwelethisi ngokuthuthukisa i-photosynthesis, ukukhula, kanye nokukhuthaza izinqubo ezihlobene nokuzinza kwesistimu. Ijenali Yesayensi Yenhlabathi Nokudla Okunomsoco Kwezitshalo 22(2):1955–1969
UZhou X, uShen H, uYan X, uMa X, uLeng J, uSun Y, uGao N (2024a) I-Acetoin ikhuthaza ukukhula kwezitshalo futhi inciphisa ukucindezeleka kukasawoti ezitshalweni ze-lettuce ngokusebenzisa izindlela ze-metabolic. I-Plants-Basel 13(23):3312
UZhou Chao, uZheng Yong, uLeng Jun, uMa Chao, uNiu Hui, uChen Yong, uYing Hui, uGao Ning (2024b) Imiphumela ye-phosphate ye-cadaverine yangaphandle ekukhuleni, i-photosynthesis, kanye nokubonakaliswa kwezakhi zofuzo ezitshalweni ze-lettuce. Ijenali YaseShayina Yesayensi Yenhlabathi Nokudla Okunomsoco Kwezitshalo 24(1):537–546
Sibonga kakhulu i-High Performance Computing Center yaseNanjing University of Technology ngokusinika izinsizakusebenza zekhompyutha.
Lolu cwaningo lusekelwe yiNational Natural Science Foundation of China (32172673 kanye no-31972503) kanye neProjekthi yePlatform yoQeqesho lokuQamba nokuQamba kwaBafundi baseNanjing University of Technology (2025DC0047, 2025DC1680, 2025DC1682).
UMnyango Wezobuchwepheshe Bezinto Eziphilayo Nobunjiniyela Bemithi, iNanjing University of Technology, eNanjing 211816, eShayina
Ilebhu Eyinhloko Yesifundazwe saseJiangsu Yokuqapha Nokulawula Ukungcola Kwemvelo Yomoya, Isikhungo Sokusungula Izinto Ezintsha Esibambisene Sobuchwepheshe Bemvelo Yomoya Nemishini, Isikole Sesayensi Yezemvelo Nobunjiniyela, Inyuvesi YaseNanjing Yesayensi Nobuchwepheshe Bolwazi, eNanjing 210044, eShayina
Conceptualization, Shanshan Li; Ukukhethwa kwedatha: Xiyue Sha, Shanshan Li, Hui Shen; Ukuhlaziya okusemthethweni, uFangfang Ren no-Xin Tao; Ukuthola imali: uNan Gao, Weiye Chen, Xiyue Sha, noZhuorui Li; Indlela yokwenza, uFangfang Ren no-Weiye Chen; Ukuphathwa kwephrojekthi, i-Nan Gao; Ukubhala - okusalungiswa kwasekuqaleni, uFangfang Ren noHui Shen; Ukubhala, ukubukeza, nokuhlela: Weishou Shen, Hui Shen, and Nan Gao.
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