Njengoba ikhiqiza amathani angaphezu kuka-700,000 ngonyaka, i-glyphosate isibulala ukhula esisetshenziswa kakhulu futhi esikhulu kunazo zonke emhlabeni.Ukumelana nokhula kanye nezinsongo ezingase zibe khona endaweni ezungezile nempilo yabantu okubangelwa ukuhlukunyezwa kwe-glyphosate kudonse ukunaka okukhulu.
Ngomhla zingama-29 kuNhlaba, ithimba likaSolwazi Guo Ruiting elivela ku-State Key Laboratory ye-Biocatalysis and Enzyme Engineering, eyasungulwa ngokuhlanganyela yiSikole Sezifundo Zokuphila sase-Hubei University kanye neminyango yezifundazwe neyongqongqoshe, lashicilela iphepha lokucwaninga lakamuva ku-Journal of Hazardous Materials, lihlaziya. ukuhlaziywa kokuqala kotshani be-barnyard.(Ukhula olubi lwe-paddy) -okutholakala ku-aldo-keto reductase AKR4C16 kanye ne-AKR4C17 kugqugquzela indlela yokusabela yokuwohloka kwe-glyphosate, futhi kuthuthukise kakhulu ukonakala kwe-glyphosate nge-AKR4C17 ngokuguqulwa kwamangqamuzana.
Ukukhula ukumelana ne-glyphosate.
Kusukela yethulwa ngawo-1970s, i-glyphosate ibilokhu idumile emhlabeni wonke, futhi kancane kancane isiphenduke umuthi oshibhe kakhulu, osetshenziswa kabanzi futhi okhiqiza kakhulu wokubulala ukhula obanzi.Idala ukuphazamiseka kwe-metabolic ezitshalweni, okuhlanganisa nokhula, ngokuvimbela ngokukhethekile i-5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), i-enzyme eyinhloko ebandakanyeka ekukhuleni kwezitshalo kanye ne-metabolism.nokufa.
Ngakho-ke, ukuzalanisa izitshalo ze-glyphosate ezingazweli kanye nokusebenzisa i-glyphosate ensimini kuyindlela ebalulekile yokulawula ukhula kwezolimo zesimanje.
Kodwa-ke, ngokusetshenziswa okubanzi nokuhlukunyezwa kwe-glyphosate, inqwaba yokhula iye yavela kancane kancane futhi yaba nokubekezelela okuphezulu kwe-glyphosate.
Ngaphezu kwalokho, izitshalo ezishintshwe izakhi zofuzo ezimelana ne-glyphosate azikwazi ukubola i-glyphosate, okuholela ekuqongeleleni nasekudluliseleni kwe-glyphosate ezitshalweni, ezingasakazeka kalula ngochungechunge lokudla futhi zibeke engozini impilo yabantu.
Ngakho-ke, kuyaphuthuma ukuthola izakhi zofuzo ezingehlisa isithunzi se-glyphosate, ukuze kutshalwe izitshalo ze-transgenic ezimelana ne-glyphosate ezinezinsalela eziphansi ze-glyphosate.
Ukuxazulula ukwakheka kwekristalu kanye nendlela yokusabela eyi-catalytic yama-enzymes atholwe esitshalweni e-glyphosate-degrading
Ngo-2019, amaqembu ocwaningo aseShayina nase-Australia akhombe ama-aldo-keto reductase amabili ehlisa izinga le-glyphosate, i-AKR4C16 kanye ne-AKR4C17, okokuqala ngqa otshanini be-barnyard obungazweli yi-glyphosate.Bangasebenzisa i-NADP+ njenge-cofactor ukuze balulaze i-glyphosate ibe i-aminomethylphosphonic acid engasinobuthi ne-glyoxylic acid.
I-AKR4C16 ne-AKR4C17 zingama-enzyme okuqala okubikiwe e-glyphosate akhiqizwa ukuvela kwemvelo kwezitshalo.Ukuze kuqhutshekwe nokuhlola indlela yamangqamuzana yokucekelwa phansi kwe-glyphosate, ithimba likaGuo Ruiting lasebenzisa i-X-ray crystallography ukuze lihlaziye ubudlelwano phakathi kwalawa ma-enzyme amabili kanye ne-cofactor ephezulu.Isakhiwo esiyinkimbinkimbi sesixazululo sembule imodi ebophezelayo ye-ternary complex ye-glyphosate, NADP+ kanye ne-AKR4C17, futhi saphakamisa indlela yokusabela eyi-catalytic ye-AKR4C16 kanye ne-AKR4C17-mediated glyphosate degradation.
Isakhiwo se-AKR4C17/NADP+/glyphosate eyinkimbinkimbi kanye nendlela yokusabela yokuwohloka kwe-glyphosate.
Ukuguqulwa kwamangqamuzana kuthuthukisa ukusebenza kahle kokuwohloka kwe-glyphosate.
Ngemva kokuthola imodeli yesakhiwo esihle enezinhlangothi ezintathu ye-AKR4C17/NADP+/glyphosate, ithimba likaSolwazi Guo Ruiting liphinde lathola iphrotheni eguquguqukayo i-AKR4C17F291D enokwenyuka okungama-70% kokuwohloka kwe-glyphosate ngokuhlaziywa kwesakhiwo se-enzyme nokuklama okunengqondo.
Ukuhlaziywa komsebenzi owehlisa i-glyphosate we-AKR4C17 mutants.
"Umsebenzi wethu wembula indlela yamangqamuzana ye-AKR4C16 kanye ne-AKR4C17 edala ukucekelwa phansi kwe-glyphosate, okubeka isisekelo esibalulekile sokuqhubeka nokulungiswa kwe-AKR4C16 ne-AKR4C17 ukuze kuthuthukiswe ukusebenza kahle kwazo kwe-glyphosate."Umbhali ohambisana naleli phepha, uSolwazi Dai Longhai wase-Hubei University uthe bakhe iphrotheni eguquguqukayo i-AKR4C17F291D ngekhono elithuthukisiwe lokuwohloka kwe-glyphosate, elihlinzeka ngethuluzi elibalulekile lokutshala izitshalo eziguqukayo ezimelana ne-glyphosate ezinezinsalela eziphansi ze-glyphosate nokusebenzisa amagciwane angama-microbial engineering. ukwehlisa i-glyphosate endaweni ezungezile.
Kubikwa ukuthi ithimba likaGuo Ruiting sekuyisikhathi eside lizibandakanyile ocwaningweni lokuhlaziywa kwesakhiwo kanye nengxoxo yemishini yama-enzyme e-biodegradation, ama-terpenoid synthases, kanye namaprotheni aqondiswe ezidakamizwa ezinto ezinobuthi neziyingozi endaweni.U-Li Hao, ongumcwaningi ohlangene u-Yang Yu kanye nomfundisi u-Hu Yumei eqenjini bangababhali bokuqala bephepha, kanti u-Guo Ruiting no-Dai Longhai bangababhali abahambisana nabo.
Isikhathi sokuthumela: Jun-02-2022